In this research study, veterinary scientists present definitive findings on the safety of oxytetracycline (OTC) administration during bovine pregnancy. Through meticulous investigation of forty crossbred dairy cows, this work addresses a critical concern in livestock management: the impact of antibiotic treatment on gestational outcomes. The research examines both 5% and 20% OTC concentrations, administered during early and late pregnancy stages, providing comprehensive data on progesterone levels and gestation periods. This essential reference delivers evidence-based insights for veterinarians, dairy farmers, and animal health professionals, demonstrating that OTC treatment can be safely implemented without compromising reproductive hormone levels or pregnancy duration. The study's robust methodology, including careful subject selection and systematic blood sampling, ensures reliable conclusions that will inform clinical practice in dairy cattle management. For professionals seeking authoritative guidance on antibiotic use during bovine pregnancy, this research provides the scientific foundation for confident decision-making in livestock healthcare.